By: Hadeel Sami Shaheen, Sawsan Atallah Oran, Mohammad Hudaib, Yasser Khalil Bustanji, Arwa Rasem Althaher
Keywords: Sedum, antiproliferative, antioxidants, essential oil, cell lines.
DOI : 10.36721/PJPS.2024.37.6.REG.1259-1270.1
Abstract: This study evaluated the antioxidant and antiproliferative effects of aqueous, ethanolic, and methanolic extracts of Sedum nicaeense flowers and leaves. The MTT assay assessed cytotoxicity against colorectal cancer cells (Caco-2, HCT-116), breast cancer cells (T47D, MCF-7) and normal fibroblasts (MRC-5), while the ferric-reducing antioxidant power (FRAP) assay measured antioxidant capacity. Essential oils from flowers and leaves were analyzed using gas chromatography-mass spectrometry (GC-MS). Methanolic and ethanolic flower extracts exhibited potent antiproliferative effects against HCT-116 cells, with IC50 values of 8.62±9.21µg/mL and 17.41±9.54µg/mL, respectively. The methanolic leaf extract showed strong activity against T47D cells (IC50: 27.33±6.43 µg/mL). The antioxidant activity of the flower’s methanolic extract and the leaves' methanolic and ethanolic extracts was comparable to ascorbic acid. GC-MS analysis revealed isopropyl myristate (21.63%) as the main component in flower oil, while 6,10,14-trimethyl-2-pentadecanone (18.09%) predominated in leaf oil. These findings highlight the therapeutic potential of S. nicaeense, with significant antiproliferative and antioxidant effects. The study provides a foundation for further exploration of its extracts and essential oils as candidates for biomedical applications.
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