By: Tanveer Hussain Bokhari, Faheem Askari Rizvi, Samina Roohi, Saira Hina, Mushtaq Ahmad, Muhammad Khalid, Munawar Iqbal
Keywords: 99mTc-Lincomycin, biodistribution, ligand/reductant ratio, E. coli, infectious lesions.
Abstract: A complex of lincomycin was synthesized with technetium-99m. The synthesis was carried out by using SnCl2.2H2O as reducing agent and ascorbic acid as stabilizer. The effect of various parameters such as amount of ligand/reducing agent, pH value and reaction time on radio labeling process was studied. The characterization of the 99mTc-Lincomycin was performed by HPLC and electrophoresis Biodistribution studies were carried out by analyzing the model of bacterial infectious rats (Sprague-Dawley). The uptake of infectious lesions at different time interval was also studied by using scintigraphic technique. The complex showed effective target to non-target ratio for various inflammatory or infectious lesions. The 99mTc-Lincomycin effective binding to living bacteria and could be used successfully as an infection imaging agent.
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