Influence of different metals on the activation and inhibition of ?-amylase from thermophilic Bacillus firmus KIBGE-IB28 Page No: 1275-1278

By: Raheela Rahmat Zohra, Shah Ali Ul Qader, Sidra Pervez, Afsheen Aman

Keywords: Metals, Inhibition, activation, amylase, Bacillus firmus.

Abstract: Thermophilic Bacillus firmus KIBGE-IB28 produced extracellular ?-amylase at temperature 70°C. Enzyme was partially purified by ammonium sulfate precipitation with 42.80 fold purification and specific activity of 1889.6 U/mg. Effects of various metals on enzyme activity were determined and it was found that enzyme activity boosted significantly in presence of Ca2+, K2+, Ba2+, Co2+ and Ni2+ whereas Zn2+, Mg2+, Na2+ and Cu2+ were found inhibitory at concentration 10mM.



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