Pakistan Journal of Pharmaceutical Sciences

Abstract: Background: Edaravone can reduce damage to brain cells in a variety of ways. Rho/ROCK signaling is involved in Alzheimer's disease. Objectives: This study uses AD cell models to explore edaravone’s effect on AD nerve synapse damage. Methods: The ROCK2 promoter luciferase reporter gene was constructed, A?25-35 was used as a processing factor and transfected into PC12 cells to construct an AD cell model which was then treated with edaravone, followed by analysis of 95 antibody (PSD95), synapse-related mRNA synapsin 1 (SYN1) level to observe its effect on Rho/ROCK signaling. Results: Edaravone can effectively inhibit the transcriptional activity of ROCK2 promoter in AD cell model, upregulate SYN1 and GAP43 protein and downregulate ROCK2. After ROCK2 overexpression, edaravone can affect SYN1 expression in AD cell model, while SYN1 expression did not change significantly after ROCK2 was silenced. Conclusion: Edaravone has a protective effect on AD nerve synapse damage and plays a role in repairing nerve synapse damage through ROCK2 signaling pathway.